Chapman Lab
Department of Neuroscience

Publications

Click here for a complete list of our publications

2024

Needham LM, Saavedra C, Rasch JK, Sole-Barber D, Schweitzer BS, Fairhall AJ, Vollbrecht CH, Wan S, Podorova Y, Bergsten AJ, Mehlenbacher B, Zhang Z, Tenbrake L, Saimi J, Kneely LC, Kirkwood JS, Pfeifer H, Chapman ER, Goldsmith RH. Label-free detection and profiling of individual solution-phase molecules. Nature. 2024 May 8. doi: 10.1038/s41586-024-07370-8. PMID: 38720082.

Wu Z, Kusick GF, Berns MMM, Raychaudhuri S, Itoh K, Walter AM, Chapman ER, Watanabe S. Synaptotagmin 7 docks synaptic vesicles to support facilitation and Doc2α-triggered asynchronous release. Elife. 2024 Mar 27;12:RP90632. doi: 10.7554/eLife.90632. PMID: 38536730.

Mehta N, Mondal S, Watson ET, Cui Q, Chapman ER. The juxtamembrane linker of synaptotagmin 1 regulates Ca2+ binding via liquid-liquid phase separation. Nat Commun. 2024 Jan 4;15(1):262. doi: 10.1038/s41467-023-44414-5. PMID: 38177243.

2023

Courtney KC, Mandal T, Mehta N, Wu L, Li Y, Das D, Cui Q, Chapman ER. Synaptotagmin-7 outperforms synaptotagmin-1 to promote the formation of large, stable fusion pores via robust membrane penetration. Nat Commun. 2023 Nov 27;14(1):7761. doi: 10.1038/s41467-023-42497-8. PMID: 38012142.

Zhang Z, Feng Z, Zhao X, Jean D, Yu Z, Chapman ER. Functionalization and higher-order organization of liposomes with DNA nanostructures. Nat Commun. 2023 Aug 29;14(1):5256. doi: 10.1038/s41467-023-41013-2. PMID: 37644062.

Vevea JD, Chapman ER. Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca2+ Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal Axons. J Neurosci. 2023 May 10;43(19):3421-3438. doi: 10.1523/JNEUROSCI.1356-22.2023. PMID: 36997314.

Ogunmowo TH, Jing H, Raychaudhuri S, Kusick GF, Imoto Y, Li S, Itoh K, Ma Y, Jafri H, Dalva MB, Chapman ER, Ha T, Watanabe S, Liu J. Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis. Nat Commun. 2023 May 20;14(1):2888. doi: 10.1038/s41467-023-38595-2. PMID: 37210439.

Bradberry MM, Peters-Clarke TM, Shishkova E, Chapman ER, Coon JJ. N-glycoproteomics of brain synapses and synaptic vesicles. Cell Rep. 2023 Apr 25;42(4):112368. doi: 10.1016/j.celrep.2023.112368. PMID: 37036808.

Zhu M, Abdulzahir A, Perkins MG, Chu CC, Krause BM, Casey C, Lennertz R, Ruhl D, Hentschke H, Nagarajan R, Chapman ER, Rudolph U, Fanselow MS, Pearce RA. Control of contextual memory through interneuronal α5-GABAA receptors. PNAS Nexus. 2023 Apr 11;2(4):pgad065. doi: 10.1093/pnasnexus/pgad065. PMID: 37056471.

Needham LM, Saavedra C, Rasch JK, Sole-Barber D, Schweitzer BS, Fairhall AJ, Vollbrecht CH, Mehlenbacher B, Zhang Z, Tenbrake L, Pfeifer H, Chapman ER, Goldsmith RH. Label-free observation of individual solution phase molecules. bioRxiv [Preprint]. 2023 Mar 25:2023.03.24.534170. doi: 10.1101/2023.03.24.534170. PMID: 36993572.

Maingi V, Zhang Z, Thachuk C, Sarraf N, Chapman ER, Rothemund PWK. Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers. Nat Commun. 2023 Mar 20;14(1):1532. doi: 10.1038/s41467-023-36996-x. PMID: 36941256.

Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER. Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion. J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2. PMID: 36732068.

Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER. Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons. Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. PMID: 36729040.

2022

Courtney KC, Wu L, Mandal T, Swift M, Zhang Z, Alaghemandi M, Wu Z, Bradberry MM, Deo C, Lavis LD, Volkmann N, Hanein D, Cui Q, Bao H, Chapman ER. The complexin C-terminal amphipathic helix stabilizes the fusion pore open state by sculpting membranes. Nat Struct Mol Biol. 2022 Feb;29(2):97-107. doi: 10.1038/s41594-021-00716-0. PMID: 35132256

Bradberry MM, Mishra S, Zhang Z, Wu L, McKetney JM, Vestling MM, Coon JJ, Chapman ER. Rapid and gentle immunopurification of brain synaptic vesicles. J Neurosci. 2022 Apr 27;42(17):3512-3522. doi: 10.1523/JNEUROSCI.2521-21.2022. PMID: 35296545

Peotter JL, Pustova I, Lettman MM, Shatadal S, Bradberry MM, Winter-Reed AD, Charan M, Sharkey EE, Alvin JR, Bren AM, Oie AK, Chapman ER, Salamat MS, Audhya A. TFG regulates secretory and endosomal sorting pathways in neurons to promote their activity and maintenance. Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2210649119. doi: 10.1073/pnas.2210649119. Epub 2022 Sep 26.PMID: 36161950

Bradberry MM, Chapman ER. All-optical monitoring of excitation-secretion coupling demonstrates that SV2A functions downstream of evoked Ca2+ entry. J Physiol. 2022 Feb;600(3):645-654. doi: 10.1113/JP282601. Epub 2022 Jan 13. PMID: 34957569

Wu Z, Ma L, Courtney NA, Zhu J, Landajuela A, Zhang Y, Chapman ER*, Karatekin E*. Polybasic patches in both C2 domains of synaptotagmin-1 are required for evoked neurotransmitter release. J Neurosci. 2022 Jul 27;42(30):5816-5829. doi: 10.1523/JNEUROSCI.1385-21.2022. PMID: 35701163. *Co-corresponding authors

2021

Courtney KC, Vevea JD, Li Y, Wu Z, Zhang Z, Chapman ER. Synaptotagmin 1 oligomerization via the juxtamembrane linker regulates spontaneous and evoked neurotransmitter release. Proc Natl Acad Sci U S A. 2021 Nov 30;118(48):e2113859118. doi: 10.1073/pnas.2113859118. PMID: 34810248

Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER. Synaptotagmin 7 is targeted to the axonal plasma membrane through γ-secretase processing to promote synaptic vesicle docking in mouse hippocampal neurons. Elife. 2021 Sep 20;10:e67261. doi: 10.7554/eLife.67261. PMID: 34543184

Wu L, Courtney KC, Chapman ER. Cholesterol stabilizes recombinant exocytic fusion pores by altering membrane bending rigidity. Biophys J. 2021 Feb 11:S0006-3495(21)00139-9. doi: 10.1016/j.bpj.2021.02.005. Online ahead of print. PMID: 33582136

Rinald JH, Vevea JD, Chapman ER. Knockoff: Druggable Cleavage of Membrane Proteins. Bio Protoc. 2021 Nov 20;11(22):e4224. doi: 10.21769/BioProtoc.4224. eCollection 2021 Nov 20. PMID: 34909445

Liu L, Courtney KC, Huth SW, Rank LA, Weisblum B, Chapman ER, Gellman SH. Beyond Amphiphilic Balance: Changing Subunit Stereochemistry Alters the Pore-Forming Activity of Nylon-3 Polymers. J Am Chem Soc. 2021 Feb 21. doi: 10.1021/jacs.0c12731. Online ahead of print. PMID: 33611913

Yang Y, Wu Z, Wang L, Zhou K, Xia K, Xiong Q, Liu L, Zhang Z, Chapman ER, Xiong Y, Melia TJ, Karatekin E, Gu H, Lin C. Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation. Nat Chem. 2021 Apr;13(4):335-342. doi: 10.1038/s41557-021-00667-5. Epub 2021 Mar 30. PMID: 33785892

Abbineni PS, Briguglio JS, Chapman ER, Holz RW, Axelrod D. VAMP2 and synaptotagmin mobility in chromaffin granule membranes: implications for regulated exocytosis. Mol Biol Cell. 2021 Dec 1:mbcE21100494. doi: 10.1091/mbc.E21-10-0494. Online ahead of print. PMID: 34851717

Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O’Toole ET, Morgan GP, Ali L, Saito T, Guillermier C, Saido TC, Steinhauser ML, Stowell MHB, Chapman ER, Contractor A, Savas JN. Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer’s Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals. Cell Syst. 2021 Feb 17;12(2):141-158.e9. doi: 10.1016/j.cels.2020.11.007. Epub 2020 Dec 15. PMID: 33326751

2020

Vevea JD, Chapman ER. Acute Disruption of the Synaptic Vesicle Membrane Protein Synaptotagmin 1 Using Knockoff in Mouse Hippocampal Neurons. Elife. 2020 Jun 9;9:e56469. doi: 10.7554/eLife.56469. PMID: 32515733

Bradberry MM, Courtney NA, Dominguez MJ, Lofquist SM, Knox AT, Sutton RB, Chapman ER. Molecular Basis for Synaptotagmin-1-Associated Neurodevelopmental Disorder. Neuron. 2020 Apr 21. pii: S0896-6273(20)30272-5. doi: 10.1016/j.neuron.2020.04.003. [Epub ahead of print] PMID: 32362337

Das D, Bao H, Courtney KC, Wu L, Chapman ER. Resolving kinetic intermediates during the regulated assembly and disassembly of fusion pores. Nat Commun. 2020 Jan 13;11(1):231. doi: 10.1038/s41467-019-14072-7. PMID: 31932584

Zhang Z, Chapman ER. Programmable Nanodisc Patterning by DNA Origami. Nano Lett. 2020 Aug 12;20(8):6032-6037. doi: 10.1021/acs.nanolett.0c02048. Epub 2020 Jul 15. PMID: 32648765

2019

Courtney NA, Bao H, Briguglio JS, Chapman ER. Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin. Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w. PMID:31501440

Ruhl DA, Bomba-Warczak E, Watson ET, Bradberry MM, Peterson TA, Basu T, Frelka A, Evans CS, Briguglio JS, Basta T, Stowell MHB, Savas JN, Roopra A, Pearce RA, Piper RC, Chapman ER. Synaptotagmin 17 controls neurite outgrowth and synaptic physiology via distinct cellular pathways. Nat Commun. 2019 Aug 6;10(1):3532. doi: 10.1038/s41467-019-11459-4. PMID:31387992

Bradberry MM, Bao H, Lou X, Chapman ER. PIP2 drives Ca2+-independent membrane penetration by the tandem C2 domain proteins synaptotagmin-1 and Doc2β. J Biol Chem. 2019 May 30. pii: jbc.RA119.007929. doi: 10.1074/jbc.RA119.007929. [Epub ahead of print] PMID: 31147445

Brose N, Brunger A, Cafiso D, Chapman ER, Diao J, Hughson FM, Jackson MB, Jahn R, Lindau M, Ma C, Rizo J, Shin YK, Söllner TH, Tamm L, Yoon TY, Zhang Y. Synaptic vesicle fusion: today and beyond. Nat Struct Mol Biol. . 2019 Aug;26(8):663-668. doi: 10.1038/s41594-019-0277-z. PMID:31384060

Sun J, Wang L, Bao H, Premi S, Das U, Chapman ER, Roy S. Functional cooperation of α-synuclein and VAMP2 in synaptic vesicle recycling. Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11113-11115. doi: 10.1073/pnas.1903049116. Epub 2019 May 20. PMID: 31110017

Harsini FM, Bui AA, Rice AM, Chebrolu S, Fuson KL, Turtoi A, Bradberry M, Chapman ER, Sutton RB. Structural Basis for the Distinct Membrane Binding Activity of the Homologous C2A Domains of Myoferlin and Dysferlin. J Mol Biol. 2019 May 17;431(11):2112-2126. doi: 10.1016/j.jmb.2019.04.006. Epub 2019 Apr 18. PMID: 31004665

Joshi DC, Zhang CL, Babujee L, Vevea JD, August BK, Sheng ZH, Chapman ER, Gomez TM, Chiu SY. Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell Rep. 2019 Oct 15;29(3):685-696.e5. doi: 10.1016/j.celrep.2019.09.012. PMID:31618636

Shen M, Wang F, Li M, Sah N, Stockton ME, Tidei JJ, Gao Y, Korabelnikov T, Kannan S, Vevea JD, Chapman ER, Bhattacharyya A, van Praag H, Zhao X. Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1-mutant mice. Nat Neurosci. 2019 Mar;22(3):386-400. doi: 10.1038/s41593-019-0338-y. Epub 2019 Feb 11. PMID: 30742117

Shivange AV, Borden PM, Muthusamy AK, Nichols AL, Bera K, Bao H, Bishara I, Jeon J, Mulcahy MJ, Cohen B, O’Riordan SL, Kim C, Dougherty DA, Chapman ER, Marvin JS, Looger LL, Lester HA. Determining the pharmacokinetics of nicotinic drugs in the endoplasmic reticulum using biosensors. J Gen Physiol. 2019 Feb 4. pii: jgp.201812201. doi: 10.1085/jgp.201812201. [Epub ahead of print] PMID: 30718376

2018

Bao H, Das D, Courtney NA, Jiang Y, Briguglio JS, Lou X, Roston D, Cui Q, Chanda B, Chapman ER. Dynamics and number of trans-SNARE complexes determine nascent fusion pore properties. Nature. 2018 Feb 8;554(7691):260-263. doi: 10.1038/nature25481. Epub 2018 Jan 31. PMID: 29420480

Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER. Excitatory and Inhibitory Neurons Utilize Different Ca2+ Sensors and Sources to Regulate Spontaneous Release.  Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 10.1016/j.neuron.2018.04.022. Epub 2018 May 10. PMID: 29754754

Xue R, Ruhl DA, Briguglio JS, Figueroa AG, Pearce RA, Chapman ER. Doc2-mediated superpriming supports synaptic augmentation. Proc Natl Acad Sci U S A. 2018 Jun 12;115(24):E5605-E5613. doi: 10.1073/pnas.1802104115. Epub 2018 May 29. PMID: 29844163

Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Müller JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL. Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nat Methods. 2018 Nov;15(11):936-939. doi: 10.1038/s41592-018-0171-3. Epub 2018 Oct 30. PMID: 30377363

Chapman ER. A Ca2+ Sensor for Exocytosis. Trends Neurosci. 2018 Jun;41(6):327-330. doi: 10.1016/j.tins.2018.03.012. PMID: 29801524

Slosarek EL, Schuh AL, Pustova I, Johnson A, Bird J, Johnson M, Frankel EB, Bhattacharya N, Hanna MG, Burke JE, Ruhl DA, Quinney K, Block S, Peotter JL, Chapman ER, Sheets MD, Butcher SE, Stagg SM, Audhya A. Pathogenic TFG Mutations Underlying Hereditary Spastic Paraplegia Impair Secretory Protein Trafficking and Axon Fasciculation. Cell Rep. 2018 Aug 28;24(9):2248-2260. doi: 10.1016/j.celrep.2018.07.081. PMID: 30157421

Bendahmane M, Bohannon KP, Bradberry MM, Rao TC, Schmidtke MW, Abbineni PS, Chon NL, Tran S, Lin H, Chapman ER, Knight JD, Anantharam A. The synaptotagmin C2B domain calcium-binding loops modulate the rate of fusion pore expansion. Mol Biol Cell. 2018 Feb 14. pii: mbc.E17-11-0623. doi: 10.1091/mbc.E17-11-0623. [Epub ahead of print] PMID: 29444959

2017

Ugur B, Bao H, Stawarski M, Duraine LR, Zuo Z, Lin YQ, Neely GG, Macleod GT, Chapman ER, Bellen HJ. The Krebs Cycle Enzyme Isocitrate Dehydrogenase 3A Couples Mitochondrial Metabolism to Synaptic Transmission. Cell Rep. 2017 Dec 26;21(13):3794-3806. doi: 10.1016/j.celrep.2017.12.005. PMID:29281828

Rao TC, Santana Rodriguez Z, Bradberry MM, Ranski AH, Dahl PJ, Schmidtke MW, Jenkins PM, Axelrod D, Chapman ER, Giovannucci DR, Anantharam A. Synaptotagmin isoforms confer distinct activation kinetics and dynamics to chromaffin cell granules. J Gen Physiol. 2017 Aug 7;149(8):763-780. doi: 10.1085/jgp.201711757. Epub 2017 Jul 7. PMID:28687607

Zurawski Z, Page B, Chicka MC, Brindley RL, Wells CA, Preininger AM, Hyde K, Gilbert JA, Cruz-Rodriguez O, Currie KPM, Chapman ER, Alford S, Hamm HE. Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes. J Biol Chem. 2017 Jul 21;292(29):12165-12177. doi: 10.1074/jbc.M116.773523. Epub 2017 May 17. PMID: 28515322

2016

Bao H, Goldschen-Ohm M, Jeggle P, Chanda B, Edwardson JM, Chapman ER. Exocytotic fusion pores are composed of both lipids and proteins. Nat Struct Mol Biol. 2016 Jan;23(1):67-73. doi: 10.1038/nsmb.3141. Epub 2015 Dec 14. PMID: 26656855

Bomba-Warczak E, Vevea JD, Brittain JM, Figueroa-Bernier A, Tepp WH, Johnson EA, Yeh FL,Chapman ER. Interneuronal Transfer and Distal Action of Tetanus Toxin and Botulinum Neurotoxins A and D in Central Neurons. Cell Rep. 2016 Aug 16;16(7):1974-87. doi: 10.1016/j.celrep.2016.06.104. Epub 2016 Aug 4. PMID: 27498860

Bai, H., Xue, R., Zhang, L., Yethiraj, A., Cui, Q. and Chapman, E.R. Different states of synaptotagmin control evoked versus spontaneous synaptic vesicle exocytosis. Nature Comm. 2016 Mar 22;7:10971. doi: 10.1038/ncomms10971. PMID: 27001899

Evans CS, He Z, Bai H, Lou X, Jeggle P, Sutton RB, Edwardson JM, Chapman ER. Functional analysis of the interface between the tandem C2-domains of synaptotagmin-1. Mol Biol Cell. 2016 Jan 20. pii: mbc.E15-07-0503. [Epub ahead of print] PMID: 26792839

McVicker DP, Awe AM, Richters KE, Wilson RL, Cowdrey DA, Hu X, Chapman ER, Dent EW. Transport of a kinesin-cargo pair along microtubules into dendritic spines undergoing synaptic plasticity. Nat Commun. 2016 Sep 23;7:12741. doi: 10.1038/ncomms12741. PMID: 27658622

Hanna MG 4th, Mela I, Wang L, Henderson RM, Chapman ER, Edwardson JM, Audhya A. Sar1 GTPase Activity Is Regulated by Membrane Curvature. J Biol Chem. 2016 Jan 15;291(3):1014-27. doi: 10.1074/jbc.M115.672287. Epub 2015 Nov 6. PMID: 26546679 paper of the week

Chang CW, Chiang CW, Gaffaney JD, Chapman ER, Jackson MB. Lipid-anchored synaptobrevin provides little or no support for exocytosis or liposome fusion. J Biol Chem. 2016 Feb 5;291(6):2848-57. doi: 10.1074/jbc.M115.701169. PMID: 26663078

Hullinger R, Li M, Wang J, Peng Y, Dowell JA, Bomba-Warczak E, Mitchell HA, Burger C, Chapman ER, Denu JM, Li L, Puglielli L. Increased expression of AT-1/SLC33A1 causes an autistic-like phenotype in mice by affecting dendritic branching and spine formation. J Exp Med. 2016 Jun 27;213(7):1267-84. doi: 10.1084/jem.20151776. Epub 2016 May 30. PMID:27242167

Dolai S, Xie L, Zhu D, Liang T, Qin T, Xie H, Kang Y, Chapman ER, Gaisano HY. Synaptotagmin-7 Functions to Replenish Insulin Granules for Exocytosis in Human Islet β-Cells. Diabetes. 2016 Jul;65(7):1962-76. doi: 10.2337/db15-1436. Epub 2016 Apr 26.PMID:27207520

2015

Evans, CE, Ruhl, DA, Chapman, ER. An Engineered metal sensor tunes the kinetics of synaptic transmission. J Neurosci. 2015 Aug;35(34):11769-79. doi: 10.1523/JNEUROSCI.1694-15.2015. PMID: 26311762

Xue, R, Gaffaney, JD, Chapman, ER. Structural elements that underlie Doc2b function during asynchronous synaptic transmission. Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):E4316-25. doi: 10.1073/pnas.1502288112. Epub 2015 Jul 20. PMID: 26195798

Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER. Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9. Nat Commun. 2015 Oct 20;6:8645. doi: 10.1038/ncomms9645. PMID: 26482442

Chang CW, Hui E, Bai J, Bruns D, Chapman ER, Jackson MB. A structural role for the synaptobrevin 2 transmembrane domain in dense-core vesicle fusion pores. J Neurosci. 2015 Apr 8;35(14):5772-80. doi: 10.1523/JNEUROSCI.3983-14.2015. PMID: 25855187

Lippmann ES, Williams CE, Ruhl DA, Estevez-Silva MC, Chapman ER, Coon JJ, Ashton RS. Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm. Stem Cell Reports. 2015 Apr 14;4(4):632-44. doi: 10.1016/j.stemcr.2015.02.018. Epub 2015 Apr 2. PMID: 25843047

2014

Liu H, Bai H, Xue R, Takahashi H, Edwardson JM, Chapman ER. Linker mutations reveal the complexity of synaptotagmin 1 action during synaptic transmission. Nat Neurosci. 2014 May;17(5):670-7. doi: 10.1038/nn.3681. Epub 2014 Mar 23. PMID: 24657966

Liu H, Bai H, Hui E, Yang L, Evans CS, Wang Z, Kwon SE, Chapman ER. Synaptotagmin 7 functions as a Ca2+-sensor for synaptic vesicle replenishment. Elife. 2014 Feb 25;3:e01524. doi: 10.7554/eLife.01524. PMID: 24569478

Gaffaney JD, Xue R, Chapman ER. Mutations that disrupt Ca²⁺-binding activity endow Doc2β with novel functional properties during synaptic transmission. Mol Biol Cell. 2014 Feb;25(4):481-94. doi: 10.1091/mbc.E13-10-0571. Epub 2013 Dec 19. PMID: 24356452

Rao TC, Passmore DR, Peleman AR, Das M, Chapman ER, Anantharam A. Distinct fusion properties of synaptotagmin-1 and synaptotagmin-7 bearing dense core granules. Mol Biol Cell. 2014 Aug 15;25(16):2416-27. doi: 10.1091/mbc.E14-02-0702. Epub 2014 Jun 18. PMID: 24943843

2013

Liu H, Chapman ER, Dean C. “Self” versus “non-self” connectivity dictates properties of synaptic transmission and plasticity. PLoS One. 2013 Apr 29;8(4):e62414. doi: 10.1371/journal.pone.0062414. Print 2013. PMID: 23658626

Beetz C, Johnson A, Schuh AL, Thakur S, Varga RE, Fothergill T, Hertel N, Bomba-Warczak E, Thiele H, Nürnberg G, Altmüller J, Saxena R, Chapman ER, Dent EW, Nürnberg P, Audhya A. Inhibition of TFG function causes hereditary axon degeneration by impairing endoplasmic reticulum structure. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5091-6. doi: 10.1073/pnas.1217197110. Epub 2013 Mar 11. PMID: 23479643

Saludes JP, Morton LA, Coulup SK, Fiorini Z, Cook BM, Beninson L, Chapman ER, Fleshner M, Yin H. Multivalency amplifies the selection and affinity of bradykinin-derived peptides for lipid nanovesicles. Mol Biosyst. 2013 Aug;9(8):2005-9. doi: 10.1039/c3mb70109c. Epub 2013 May 28. PMID: 23715428

Morton LA, Yang H, Saludes JP, Fiorini Z, Beninson L, Chapman ER, Fleshner M, Xue D, Yin H. MARCKS-ED peptide as a curvature and lipid sensor. ACS Chem Biol. 2013 Jan 18;8(1):218-25. doi: 10.1021/cb300429e. Epub 2012 Oct 29. PMID: 23075500

2012

Kwon SE, Chapman ER. Glycosylation is dispensable for sorting of synaptotagmin 1 but is critical for targeting of SV2 and synaptophysin to recycling synaptic vesicles. J Biol Chem. 2012 Oct 12;287(42):35658-68. PMID: 22908222

Wu Y, Gu Y, Morphew MK, Yao J, Yeh FL, Dong M, Chapman ER. All three components of the neuronal SNARE complex contribute to secretory vesicle docking. J Cell Biol. 2012 Aug 6;198(3):323-30. PMID: 22869597

Sun S, Tepp WH, Johnson EA, Chapman ER. Botulinum Neurotoxins B and E Translocate at Different Rates and Exhibit Divergent Responses to GT1b and Low pH. Biochemistry. 2012 Jul 2. [Epub ahead of print] PMID: 22720883

Dean C, Liu H, Staudt T, Stahlberg MA, Vingill S, Bückers J, Kamin D, Engelhardt J, Jackson MB, Hell SW, Chapman ER. Distinct subsets of Syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity. J Neurosci. 2012 Apr 18;32(16):5398-413. PMID: 22514304

Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER. Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen. Mol Biol Cell. 2012 May;23(9) 1715-27. Epub 2012 Mar 7. PMID: 22398727

Striegel, A.R., Biela, L.M., Evans, C., Wang, Z., Chapman, E.R., and Reist, N.E. Calcium binding by synaptotagmin’s C2A domain is an essential element of the electrostatic switch that triggers synchronous synaptic transmission. J Neurosci. 2012 Jan 25;32(4):1253-60. doi: 10.1523/JNEUROSCI.4652-11.2012. PMID: 22279210

Saludes JP, Morton LA, Ghosh N, Beninson L, Chapman ER, Fleshner M, Yin H. Detection of Highly Curved Membrane Surfaces Using a Cyclic Peptide Derived from Synaptotagmin-I. ACS Chem Biol. 2012 Jul 6. [Epub ahead of print] PMID: 22769435

2011

Yao J, Kwon SE, Gaffaney JD, Dunning FM, Chapman ER. Uncoupling the roles of synaptotagmin I during endo- and exocytosis of synaptic vesicles. Nat Neurosci. 2011 Dec 25;15(2):243-9. doi: 10.1038/nn.3013. PMID: 22197832

Yao J, Gaffaney JD, Kwon SE, Chapman ER. Doc2 is a Ca2+ sensor required for asynchronous neurotransmitter release. Cell. 2011 Oct 28;147(3):666-77. PMID: 22036572

Kwon SE, Chapman ER. Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron. 2011 Jun 9;70(5):847-54. doi: 10.1016/j.neuron.2011.04.001. PMID: 21658579

Hui E, Gaffaney JD, Wang Z, Johnson CP, Evans CS, Chapman ER. Mechanism and function of synaptotagmin-mediated membrane apposition. Nat Struct Mol Biol. 2011 Jun 5;18(7):813-21. doi: 10.1038/nsmb.2075. PMID: 21642967

Wang Z, Liu H, Gu Y, Chapman ER. Reconstituted synaptotagmin I mediates vesicle docking, priming, and fusion. J Cell Biol. 2011 Dec 26;195(7):1159-70. Epub 2011 Dec 19. PMID: 22184197

Yeh FL, Zhu Y, Tepp WH, Johnson EA, Bertics PJ, Chapman ER. Retargeted clostridial neurotoxins as novel agents for treating chronic diseases. Biochemistry. 2011 Dec 6;50(48):10419-21. Epub 2011 Nov 7. PMID: 22047069

Wu, Y., Ma, L., Cheley, S., Bayley, H., Cui, Q. and Chapman, E.R. Permeation of styryl dyes through nanometer-scale pores in membranes. Biochemistry. 2011 Sep 6;50(35):7493-502. doi: 10.1021/bi2006288. Epub 2011 Aug 16. PMID: 21815625

Sun S, Suresh S, Liu H, Tepp WH, Johnson EA, Edwardson JM, Chapman ER. Receptor binding enables botulinum neurotoxin B to sense low pH for translocation channel assembly. Cell Host Microbe. 2011 Sep 15;10(3):237-47. doi: 10.1016/j.chom.2011.06.012. PMID: 21925111

Zhang, Z., Wu, Y., Wang, Z., Dunning, F.M., Rehfuss, J., Ramanan, D., Chapman, E.R., and Jackson, M.B. Release mode of large and small dense-core vesicles specified by different synaptotagmin isoforms in PC12 cells. Mol Biol Cell. 2011 Jul 1;22(13):2324-36. doi: 10.1091/mbc.E11-02-0159. Epub 2011 May 5. PMID: 21551071

Wang, D., Zhang, Z., Dong, M., Sun, S., Chapman, E.R., and Jackson, M.B. Syntaxin requirement for Ca2+-triggered exocytosis in neurons and endocrine cells demonstrated with an engineered neurotoxin. Biochemistry. 2011 Apr 12;50(14):2711-3. doi: 10.1021/bi200290p. Epub 2011 Mar 18. PMID: 21401123

Paddock, B.E., Wang, Z., Biela, L.M., Chen, K., Getzy, M.D., Striegel, A., Richmond, J.E., Chapman, E.R., Featherstone, D.E. and Reist, N.E. Membrane penetration by synaptotagmin is required for coupling calcium binding to vesicle fusion in vivo. J Neurosci. 2011 Feb 9;31(6):2248-57. doi: 10.1523/JNEUROSCI.3153-09.2011. PMID: 21307261

Mayers JR, Fyfe I, Schuh AL, Chapman ER, Edwardson JM, Audhya A. ESCRT-0 assembles as a heterotetrameric complex on membranes and binds multiple ubiquitinylated cargoes simultaneously. J Biol Chem. 2011 Mar 18;286(11):9636-45. doi: 10.1074/jbc.M110.185363. Epub 2010 Dec 30. PMID: 21193406

2010

Yeh FL, Dong M, Yao J, Tepp WH, Lin G, Johnson EA, Chapman ER. SV2 mediates entry of tetanus neurotoxin into central neurons. PLoS Pathog. 2010 Nov 24;6(11):e1001207. doi: 10.1371/journal.ppat.1001207. PMID: 21124874

Johnson CP, Chapman ER. Otoferlin is a calcium sensor that directly regulates SNARE-mediated membrane fusion. J Cell Biol. 2010 Oct 4;191(1):187-97. doi: 10.1083/jcb.201002089. PMID: 20921140

Wang Z, Chapman ER. Rat and Drosophila synaptotagmin 4 have opposite effects during SNARE-catalyzed membrane fusion. J Biol Chem. 2010 Oct 1;285(40):30759-66. doi: 10.1074/jbc.M110.137745. Epub 2010 Aug 5. PMID: 20688915

Zhang Z, Hui E, Chapman ER, Jackson MB. Regulation of exocytosis and fusion pores by synaptotagmin-effector interactions. Mol Biol Cell. 2010 Aug 15;21(16):2821-31. doi: 10.1091/mbc.E10-04-0285. Epub 2010 Jun 23. PMID: 20573977

Beurg M, Michalski N, Safieddine S, Bouleau Y, Schneggenburger R, Chapman ER, Petit C, Dulon D. Control of exocytosis by synaptotagmins and otoferlin in auditory hair cells. J Neurosci. 2010 Oct 6;30(40):13281-90. doi: 10.1523/JNEUROSCI.2528-10.2010. PMID: 20926654

Stenmark P, Dong M, Dupuy J, Chapman ER, Stevens RC. Crystal structure of the botulinum neurotoxin type G binding domain: insight into cell surface binding. J Mol Biol. 2010 Apr 16;397(5):1287-97. doi: 10.1016/j.jmb.2010.02.041. Epub 2010 Feb 26. PMID: 20219474

Arthur CP, Dean C, Pagratis M, Chapman ER, Stowell MH. Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons. Neuroscience. 2010 Apr 28;167(1):135-42. doi: 10.1016/j.neuroscience.2010.01.056. Epub 2010 Feb 4. PMID: 20138128